Where is the aqueous humor located?

• A. Anterior and posterior chambers
• B. Posterior chamber only
• C. Anterior chamber only
• D. Vitreous body

Answer: (A)

Aqueous humor lives in the eye’s anterior segment, specifically in both the posterior chamber (between the iris and lens) and the anterior chamber (between the cornea and iris). It is produced by the ciliary processes in the posterior chamber, then flows through the pupil into the anterior chamber, where it drains to maintain intraocular pressure. The vitreous body is a separate, gel-like chamber behind the lens that contains vitreous humor, not aqueous. So the best answer is that the aqueous humor is located in both the posterior and anterior chambers.